Strain engineering for improved expression of recombinant proteins in bacteria T Makino, G Skretas, G Georgiou Microbial cell factories 10, 1-10, 2011 | 249 | 2011 |
Regulation of protein activity with small‐molecule‐controlled inteins G Skretas, DW Wood Protein Science 14 (2), 523-532, 2005 | 134 | 2005 |
Efficient production of membrane‐integrated and detergent‐soluble G protein‐coupled receptors in Escherichia coli AJ Link, G Skretas, EM Strauch, NS Chari, G Georgiou Protein Science 17 (10), 1857-1863, 2008 | 89 | 2008 |
Discovery and characterization of a thermostable and highly halotolerant GH5 cellulase from an icelandic hot spring isolate D Zarafeta, D Kissas, C Sayer, SR Gudbergsdottir, E Ladoukakis, ... PLoS One 11 (1), e0146454, 2016 | 76 | 2016 |
Comprehensive engineering of Escherichia coli for enhanced expression of IgG antibodies T Makino, G Skretas, TH Kang, G Georgiou Metabolic engineering 13 (2), 241-251, 2011 | 74 | 2011 |
Metagenomic mining for thermostable esterolytic enzymes uncovers a new family of bacterial esterases D Zarafeta, D Moschidi, E Ladoukakis, S Gavrilov, ED Chrysina, ... Scientific reports 6 (1), 38886, 2016 | 67 | 2016 |
Engineered chimeric enzymes as tools for drug discovery: generating reliable bacterial screens for the detection, discovery, and assessment of estrogen receptor modulators G Skretas, AK Meligova, C Villalonga-Barber, DJ Mitsiou, MN Alexis, ... Journal of the American Chemical Society 129 (27), 8443-8457, 2007 | 66 | 2007 |
A bacterial biosensor of endocrine modulators G Skretas, DW Wood Journal of molecular biology 349 (3), 464-474, 2005 | 55 | 2005 |
Genetic analysis of G protein‐coupled receptor expression in Escherichia coli: inhibitory role of DnaJ on the membrane integration of the human central cannabinoid receptor G Skretas, G Georgiou Biotechnology and bioengineering 102 (2), 357-367, 2009 | 54 | 2009 |
Simple genetic selection protocol for isolation of overexpressed genes that enhance accumulation of membrane-integrated human G protein-coupled receptors in Escherichia coli G Skretas, G Georgiou Applied and environmental microbiology 76 (17), 5852-5859, 2010 | 47 | 2010 |
Discovering novel hydrolases from hot environments R Wohlgemuth, J Littlechild, D Monti, K Schnorr, T van Rossum, B Siebers, ... Biotechnology advances 36 (8), 2077-2100, 2018 | 44 | 2018 |
Expression of active human sialyltransferase ST6GalNAcI in Escherichia coli G Skretas, S Carroll, S DeFrees, MF Schwartz, KF Johnson, G Georgiou Microbial cell factories 8, 1-11, 2009 | 43 | 2009 |
Development of Escherichia coli Strains That Withstand Membrane Protein-Induced Toxicity and Achieve High-Level Recombinant Membrane Protein Production D Gialama, K Kostelidou, M Michou, DC Delivoria, FN Kolisis, G Skretas ACS synthetic biology 6 (2), 284-300, 2017 | 41 | 2017 |
Development of a four-enzyme magnetic nanobiocatalyst for multi-step cascade reactions A Giannakopoulou, M Patila, K Spyrou, N Chalmpes, D Zarafeta, ... Catalysts 9 (12), 995, 2019 | 33 | 2019 |
Multi-copy genes that enhance the yield of mammalian G protein-coupled receptors in Escherichia coli G Skretas, T Makino, N Varadarajan, M Pogson, G Georgiou Metabolic engineering 14 (5), 591-602, 2012 | 32 | 2012 |
ANASTASIA: an automated metagenomic analysis pipeline for novel enzyme discovery exploiting next generation sequencing data T Koutsandreas, E Ladoukakis, E Pilalis, D Zarafeta, FN Kolisis, G Skretas, ... Frontiers in genetics 10, 434571, 2019 | 27 | 2019 |
An integrated bacterial system for the discovery of chemical rescuers of disease-associated protein misfolding I Matis, DC Delivoria, B Mavroidi, N Papaevgeniou, S Panoutsou, ... Nature Biomedical Engineering 1 (10), 838-852, 2017 | 25 | 2017 |
XynDZ5: a new thermostable GH10 xylanase D Zarafeta, AP Galanopoulou, ME Leni, SI Kaili, MS Chegkazi, ... Frontiers in microbiology 11, 498087, 2020 | 23 | 2020 |
Optimization of Recombinant Membrane Protein Production in the Engineered Escherichia coli Strains SuptoxD and SuptoxR M Michou, C Kapsalis, C Pliotas, G Skretas ACS Synthetic Biology 8 (7), 1631-1641, 2019 | 23 | 2019 |
Functional requirements for DjlA-and RraA-mediated enhancement of recombinant membrane protein production in the engineered Escherichia coli strains SuptoxD and SuptoxR D Gialama, DC Delivoria, M Michou, A Giannakopoulou, G Skretas Journal of Molecular Biology 429 (12), 1800-1816, 2017 | 22 | 2017 |